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Aug 18, 2015 · Construction and characterization of stable cell lines. The HEK-PAR OFF cell line, transgenic for paraben-repressible secreted alkaline phosphatase (SEAP) expression, was constructed by co-transfection of HEK-293 cells with a 10:5:1 (w/w/w) mixture of pWH8 (P SV40-PMA-pA), pWH10 (P PMA-SEAP-pA) and pZeoSV2(+) (P SV40-zeo-pA), followed by selection in culture medium containing …Learn More
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Single-base mapping of m6A by an antibody-independent ...
To the best of our knowledge, this is the first batch of individual m 6 A sites confirmed by two independent methods. One validated m 6 A site (chr1: 29070177; Fig. 3B ) is located at 343 nt upstream from the stop codon of m 6 A reader protein YTHDF2, suggesting a self-regulating feedback loop mediated by RNA modification.Learn More
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